Seasonal variation is associated with coordinated changes in the milk metabolome within the Parmigiano Reggiano PDO production system

Published: 28 July 2026| Version 1 | DOI: 10.17632/kpgdvh9dkg.1
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Supplemental material. This is an excel file containing the following sheets: sheet a) compounds putatively annotated according to the UHPLC-HRMS workflow against the database BMDB in the different milk samples; sheet b) hierarchical clustering and PLS-DA graphs built considering all the annotated metabolites and the five seasons under investigations; sheet c) most significant and discriminant seasonal biomarkers characterizing each main cluster; sheet d) Significant metabolites resulting from the Volcano Plot analysis when considering the pairwise comparison Summer 2024 vs Summer 2023; sheet e) Permutation plot (n = 100 random permutation) for the OPLS-DA prediction model discriminating Summer 2024 and Summer 2023; sheet f) Most discriminant (VIP) and significant (Volcano Plot and AUC from ROC) metabolites characterizing the milk metabolome as a function of the different summer seasons.

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Food Chemistry, Dairy Chemistry, Milk Quality

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